John F. Guzowski

Picture of John F. Guzowski
Associate Professor, Neurobiology and Behavior
School of Biological Sciences
James L. McGaugh Chair in the Neurobiology of Learning & Memory (rotating chair; 2010-2015)
Ph.D., University of California, Irvine, 1994, Molecular Biology and Biochemistry
B.S., Oakland University, 1986, Biochemistry
Phone: (949) 824-0259
University of California, Irvine
108 Bonney Research Lab
Mail Code: 3800
Irvine, CA 92697
Research Interests
learning, memory, synaptic plasticity, hippocampus, gene expression, neuroinflammation
Research Abstract
Research in our laboratory is focused on providing a more complete understanding of the cellular and molecular mechanisms involved in cognition and memory storage in the mammalian brain. Specifically, we are interested in the role that immediate-early genes (IEGs) induced by neural activated associated with learning play in stabilizing neural networks to ultimately encode long-term memories. These studies require a multidisciplinary approach, using methods from molecular biology, biochemistry, and systems- and behavioral neuroscience. Our work can be divided broadly into 3 main areas: 1) Determining the molecular and systems interactions regulating IEG expression following learning, 2) Identifying the role of different IEGs to neuroplastic processes, and 3) Using advanced IEG imaging methods to define neuronal population interactions involved in learning and memory. This research will increase understanding of the neurobiological bases of memory, and may provide the basis for future advances in the diagnosis and treatment of memory disorders in humans.
46: Lomeli N, Di K, Czerniawski J, Guzowski JF, and Bota DA. (2017) Cisplatin-induced mitochondrial dysfunction is associated with impaired cognitive function in rats. Free Radic Biol Med, 102:274-286.

45: Witharana WK, Cardiff J, Chawla MK, Xie JY, Alme CB, Eckert M, Lapointe V, Demchuk A, Maurer AP, Trivedi V, Sutherland RJ, Guzowski JF, Barnes CA, and McNaughton BL. (2016) Nonuniform allocation of hippocampal neurons to place fields across all hippocampal subfields. Hippocampus, 26(10):1328-44.

44: Czerniawski J, Miyashita T, Lewandowski G, and Guzowski JF. (2015) Systemic lipopolysaccharide administration impairs retrieval of context-object discrimination, but not spatial, memory: Evidence for selective disruption of specific hippocampus-dependent memory functions during acute neuroinflammation. Brain, Behavior, and Immunity, 44:159-166.

43: Pevzner A and Guzowski JF. (2014) Immediate-early gene transcriptional activation in hippocampus CA1 and CA3 does not accurately reflect rapid, pattern completion based retrieval of context memory. Learning & Memory, 22(1): 1-5.

42: Alaghband Y, O’Dell SJ, Azarnia AJ, Guzowski JF, and Marshall JF. (2014) Retrieval-induced NMDA receptor-dependent Arc expression in two models of cocaine-cue memory. Neurobiology of Learning and Memory, 116:79-89.

41: Czerniawski J and Guzowski JF. (2014) Acute neuroinflammation impairs context discrimination memory and impairs pattern separation processes in hippocampus. The Journal of Neuroscience, 34(37): 12470-12480.

40: Pevzner A, Miyashita T, Schiffman AJ, Guzowski JF. Temporal dynamics of Arc gene induction in hippocampus: relationship to context memory formation.
Neurobiol Learn Mem. 2012 Mar;97(3):313-20. doi: 10.1016/j.nlm.2012.02.004. Epub ; 2012 Feb 27. PubMed PMID: 22390855.

39: Kubik S, Miyashita T, Kubik-Zahorodna A, Guzowski JF. Loss of
activity-dependent Arc gene expression in the retrosplenial cortex after hippocampal inactivation: interaction in a higher-order memory circuit. Neurobiol Learn Mem. 2012 Jan;97(1):124-31. doi: 10.1016/j.nlm.2011.10.004. Epub 2011 Nov11. PubMed PMID: 22100445.

38: Miyashita T, Kubik S, Haghighi N, Steward O, Guzowski JF. Rapid activation of plasticity-associated gene transcription in hippocampal neurons provides a mechanism for encoding of one-trial experience. J Neurosci. 2009 Jan28;29(4):898-906. doi:10.1523/JNEUROSCI.4588-08.2009. PubMed PMID: 19176799;PubMed Central PMCID: PMC2749324.

37: Bramham CR, Worley PF, Moore MJ, Guzowski JF. The immediate early gene arc/arg3.1: regulation, mechanisms, and function. J Neurosci. 2008 Nov 12;28(46):11760-7. doi: 10.1523/JNEUROSCI.3864-08.2008. Review. PubMed PMID:19005037; PubMed Central PMCID: PMC2615463.

36: Alkire MT, Guzowski JF. Hypothesis: suppression of memory protein formation underlies anesthetic-induced amnesia. Anesthesiology. 2008 Nov;109(5):768-70.
doi: 10.1097/ALN.0b013e31818aa6f2. Review. PubMed PMID: 18946284; PubMed Central PMCID: PMC2699485.

35: Guzowski JF, Worley PF. Cellular compartment analysis of temporal activity by fluorescence in situ hybridization (catFISH). Curr Protoc Neurosci. 2001 Aug;Chapter 1:Unit 1.8. doi: 10.1002/0471142301.ns0108s15. PubMed PMID: 18428454.

34: Kubik S, Miyashita T, Guzowski JF. Using immediate-early genes to map hippocampal subregional functions. Learn Mem. 2007 Nov 14;14(11):758-70. Print 2007 Nov. Review. PubMed PMID: 18007019.

33: Miyashita T, Kubik S, Lewandowski G, Guzowski JF. Networks of neurons, networks of genes: an integrated view of memory consolidation. Neurobiol Learn Mem. 2008 Mar;89(3):269-84. Epub 2007 Oct 10. Review. PubMed PMID: 17931913; PubMed Central PMCID: PMC2293276.

32: Lin G, Chawla MK, Olson K, Barnes CA, Guzowski JF, Bjornsson C, Shain W, Roysam B. A multi-model approach to simultaneous segmentation and classification of heterogeneous populations of cell nuclei in 3D confocal microscope images.
Cytometry A. 2007 Sep;71(9):724-36. PubMed PMID: 17654650.

31: Steward O, Huang F, Guzowski JF. A form of perforant path LTP can occur without ERK1/2 phosphorylation or immediate early gene induction. Learn Mem. 2007; Jun 11;14(6):433-45. Print 2007 Jun. PubMed PMID: 17562895; PubMed Central PMCID:

30: Han JH, Kushner SA, Yiu AP, Cole CJ, Matynia A, Brown RA, Neve RL, Guzowski JF, Silva AJ, Josselyn SA. Neuronal competition and selection during memory
formation. Science. 2007 Apr 20;316(5823):457-60. PubMed PMID: 17446403.

29: Fletcher BR, Baxter MG, Guzowski JF, Shapiro ML, Rapp PR. Selective cholinergic depletion of the hippocampus spares both behaviorally induced Arc transcription and spatial learning and memory. Hippocampus. 2007;17(3):227-34.
PubMed PMID: 17286278.

28: Sutherland VL, Timlin JA, Nieman LT, Guzowski JF, Chawla MK, Worley PF, Roysam B, McNaughton BL, Sinclair MB, Barnes CA. Advanced imaging of multiple mRNAs in brain tissue using a custom hyperspectral imager and multivariate curve
resolution. J Neurosci Methods. 2007 Feb 15;160(1):144-8. Epub 2006 Oct 17.
PubMed PMID: 17049619; PubMed Central PMCID: PMC1815393.

27: Vazdarjanova A, Ramirez-Amaya V, Insel N, Plummer TK, Rosi S, Chowdhury S,Mikhael D, Worley PF, Guzowski JF, Barnes CA. Spatial exploration induces ARC, a
plasticity-related immediate-early gene, only in calcium/calmodulin-dependent protein kinase II-positive principal excitatory and inhibitory neurons of the rat
forebrain. J Comp Neurol. 2006 Sep 20;498(3):317-29. PubMed PMID: 16871537.

26: Tafoya LC, Mameli M, Miyashita T, Guzowski JF, Valenzuela CF, Wilson MC.
Expression and function of SNAP-25 as a universal SNARE component in GABAergic neurons. J Neurosci. 2006 Jul 26;26(30):7826-38. Erratum in: J Neurosci. 2006 Aug
23;26(34):8875. PubMed PMID: 16870728.

25: Guzowski JF, Miyashita T, Chawla MK, Sanderson J, Maes LI, Houston FP, Lipa P, McNaughton BL, Worley PF, Barnes CA. Recent behavioral history modifies
coupling between cell activity and Arc gene transcription in hippocampal CA1 neurons. Proc Natl Acad Sci U S A. 2006 Jan 24;103(4):1077-82. Epub 2006 Jan 13.
PubMed PMID: 16415163; PubMed Central PMCID: PMC1347968.

24: Guzowski JF, Timlin JA, Roysam B, McNaughton BL, Worley PF, Barnes CA.
Mapping behaviorally relevant neural circuits with immediate-early gene expression. Curr Opin Neurobiol. 2005 Oct;15(5):599-606. Review. PubMed PMID:

23: McIntyre CK, Miyashita T, Setlow B, Marjon KD, Steward O, Guzowski JF, McGaugh JL. Memory-influencing intra-basolateral amygdala drug infusions modulate expression of Arc protein in the hippocampus. Proc Natl Acad Sci U S A. 2005 Jul 26;102(30):10718-23. Epub 2005 Jul 14. PubMed PMID: 16020527; PubMed Central PMCID: PMC1175582.

22: Zhang WP, Guzowski JF, Thomas SA. Mapping neuronal activation and the influence of adrenergic signaling during contextual memory retrieval. Learn Mem. 2005 May-Jun;12(3):239-47. PubMed PMID: 15930502; PubMed Central PMCID:

21: Chawla MK, Guzowski JF, Ramirez-Amaya V, Lipa P, Hoffman KL, Marriott LK,Worley PF, McNaughton BL, Barnes CA. Sparse, environmentally selective expression of Arc RNA in the upper blade of the rodent fascia dentata by brief spatial experience. Hippocampus. 2005;15(5):579-86. PubMed PMID: 15920719.

20: Lin G, Chawla MK, Olson K, Guzowski JF, Barnes CA, Roysam B. Hierarchical,model-based merging of multiple fragments for improved three-dimensional segmentation of nuclei. Cytometry A. 2005;63(1):20-33. PubMed PMID: 15584021.

19: Guzowski JF, Knierim JJ, Moser EI. Ensemble dynamics of hippocampal regions CA3 and CA1. Neuron. 2004 Nov 18;44(4):581-4. Review. PubMed PMID: 15541306.

18: Chawla MK, Lin G, Olson K, Vazdarjanova A, Burke SN, McNaughton BL, Worley PF, Guzowski JF, Roysam B, Barnes CA. 3D-catFISH: a system for automated quantitative three-dimensional compartmental analysis of temporal gene transcription activity imaged by fluorescence in situ hybridization. J Neurosci Methods. 2004 Oct 15;139(1):13-24. PubMed PMID: 15351517.

17: Vazdarjanova A, Guzowski JF. Differences in hippocampal neuronal population responses to modifications of an environmental context: evidence for distinct, yet complementary, functions of CA3 and CA1 ensembles. J Neurosci. 2004 Jul
21;24(29):6489-96. PubMed PMID: 15269259.

16: Dickey CA, Gordon MN, Mason JE, Wilson NJ, Diamond DM, Guzowski JF, Morgan D. Amyloid suppresses induction of genes critical for memory consolidation in APP + PS1 transgenic mice. J Neurochem. 2004 Jan;88(2):434-42. PubMed PMID: 14690531.

15: Lin G, Adiga U, Olson K, Guzowski JF, Barnes CA, Roysam B. A hybrid 3D watershed algorithm incorporating gradient cues and object models for automatic segmentation of nuclei in confocal image stacks. Cytometry A. 2003 Nov;56(1):23-36. PubMed PMID: 14566936.

14: Vazdarjanova A, McNaughton BL, Barnes CA, Worley PF, Guzowski JF.
Experience-dependent coincident expression of the effector immediate-early genes arc and Homer 1a in hippocampal and neocortical neuronal networks. J Neurosci.2002 Dec 1;22(23):10067-71. PubMed PMID: 12451105.

13: Guzowski JF. Insights into immediate-early gene function in hippocampal memory consolidation using antisense oligonucleotide and fluorescent imaging approaches. Hippocampus. 2002;12(1):86-104. Review. PubMed PMID: 11918292.

12: Bottai D, Guzowski JF, Schwarz MK, Kang SH, Xiao B, Lanahan A, Worley PF, Seeburg PH. Synaptic activity-induced conversion of intronic to exonic sequence in Homer 1 immediate early gene expression. J Neurosci. 2002 Jan 1;22(1):167-75.
PubMed PMID: 11756499.

11: Guzowski JF, McNaughton BL, Barnes CA, Worley PF. Imaging neural activity with temporal and cellular resolution using FISH. Curr Opin Neurobiol. 2001 Oct;11(5):579-84. Review. PubMed PMID: 11595491.

10: Guzowski JF, Setlow B, Wagner EK, McGaugh JL. Experience-dependent gene expression in the rat hippocampus after spatial learning: a comparison of the immediate-early genes Arc, c-fos, and zif268. J Neurosci. 2001 Jul
15;21(14):5089-98. PubMed PMID: 11438584.

09: Redish AD, Battaglia FP, Chawla MK, Ekstrom AD, Gerrard JL, Lipa P, Rosenzweig ES, Worley PF, Guzowski JF, McNaughton BL, Barnes CA. Independence of firing correlates of anatomically proximate hippocampal pyramidal cells. J Neurosci. 2001 Mar 1;21(5):RC134. PubMed PMID: 11222672.

08: Guzowski JF, Lyford GL, Stevenson GD, Houston FP, McGaugh JL, Worley PF, Barnes CA. Inhibition of activity-dependent arc protein expression in the rat hippocampus impairs the maintenance of long-term potentiation and the consolidation of long-term memory. J Neurosci. 2000 Jun 1;20(11):3993-4001.
PubMed PMID: 10818134.

07: Guzowski JF, McNaughton BL, Barnes CA, Worley PF. Environment-specific expression of the immediate-early gene Arc in hippocampal neuronal ensembles. Nat Neurosci. 1999 Dec;2(12):1120-4. PubMed PMID: 10570490.

06: Guzowski JF, McGaugh JL. Antisense oligodeoxynucleotide-mediated disruption of hippocampal cAMP response element binding protein levels impairs consolidation of memory for water maze training. Proc Natl Acad Sci U S A. 1997 Mar18;94(6):2693-8. PubMed PMID: 9122258; PubMed Central PMCID: PMC20151.

05: Wagner EK, Guzowski JF, Singh J. Transcription of the herpes simplex virus genome during productive and latent infection. Prog Nucleic Acid Res Mol Biol. 1995;51:123-65. Review. PubMed PMID: 7659774.

04: Guzowski JF, Singh J, Wagner EK. Transcriptional activation of the herpes simplex virus type 1 UL38 promoter conferred by the cis-acting downstream activation sequence is mediated by a cellular transcription factor. J Virol. 1994 Dec;68(12):7774-89. PubMed PMID: 7966567; PubMed Central PMCID: PMC237239.

03: Huang CJ, Goodart SA, Rice MK, Guzowski JF, Wagner EK. Mutational analysis of sequences downstream of the TATA box of the herpes simplex virus type 1 major capsid protein (VP5/UL19) promoter. J Virol. 1993 Sep;67(9):5109-16. PubMed PMID: 8394439; PubMed Central PMCID: PMC237908.

02: Guzowski JF, Wagner EK. Mutational analysis of the herpes simplex virus type 1 strict late UL38 promoter/leader reveals two regions critical in transcriptional regulation. J Virol. 1993 Sep;67(9):5098-108. PubMed PMID:
8394438; PubMed Central PMCID: PMC237907.

01: Goodart SA, Guzowski JF, Rice MK, Wagner EK. Effect of genomic location on expression of beta-galactosidase mRNA controlled by the herpes simplex virus type 1 UL38 promoter. J Virol. 1992 May;66(5):2973-81. PubMed PMID: 1313912; PubMed Central PMCID: PMC241056.
Other Experience
Graduate Programs
Interdepartmental Neuroscience Program
Neurobiology and Behavior
Research Centers
Center for the Neurobiology of Learning & Memory
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