Yi-quan Tang
Associate Specialist, Molecular Biology and Biochemistry
School of Biological Sciences
School of Biological Sciences
Ph.D. equivalent, Shanghai Institute of Materia Medica, Chinese Academy of Science, 1982, Chemistry & Biochemistry
University of California, Irvine
1421 McGaugh Hall
Mail Code: 3900
Irvine, CA 92697
1421 McGaugh Hall
Mail Code: 3900
Irvine, CA 92697
Research Interests
Purification and structural determination of peptides and proteins, post translational modification of proteins, protein chemistry
Appointments
Department of Pathology, University of California Irvine (1990), Studies on cell mediated and humoral host defense system.
Research Abstract
Organic synthesis of pharmaceuticals from Chinese medicine. Methods of high performance liquid chromatography. Purification and molecular characterization of neuroactive peptides from the Chinese amphibian skin. Cell mediated and humoral host defense systems. Purification of growth factors with haematopoietic activity from microvascular endothelial cells. Cell calture of platelets, monocytes. Peptide and protein methylation. Techniques used: HPLC, FPLC, MALDI-TOF and ES mass spectroscopy, Edman degradation, amino acid analysis, gel electrophoresis, antimicrobial assays, immunoblot, Western blot, cell culture, and radioisotope assays.
Publications
PUBLICATIONS LIST
1. Tang, Y.Q., M. R. Yeaman, and M. E. Selsted. Antimicrobial peptides from human platelets. Infec. Immun. 70: 6524-6533(2002)
2. Tran, D., P. A. Tran, Y. Q. Tang, J. Yuan, T. Cole, and M. E. Selsted. Homodimeric q-defensins from Rhesus macaque leukocytes. J. Biol. Chem. 277: 3079-3084 (2002).
3 Tang, Y.-Q., J. Yuan, G. Osapay, K. Osapay, D. Tran, C.J. Miller, A.J.
Ouellette and M.E. Selsted. A cyclic antimicrobial peptide produced in primate leukocytes by the ligation of two truncated a-defensins. Science, 286: 498-502 (1999).
4. Tang, Y.Q., J. Yuan, C. J. Miller, and M.E. Selsted. Isolation,
characterization, cDNA cloning and antimicrobial properties of two distinct subfamilies from Rhesus macaque leukocytes. Infec. Immun., 67: 6139-6144 (1999).
5. Yeaman, M.R., Y.Q. Tang, A.J. Shen, A.S. Bayer, and M.E. Selsted. Purification and in vitro avtivities of rabbit platelet microbicidal proteins. Infec. Immun., 65: 1023-1031 (1997).
6. Van Abel, R.J., Y.Q. Tang, V.S.V. Rao, C.H. Dobbs, D. Tran, G. Barany, and M.E. Selsted. Synthesis and characterization of indolicidin, a tryptophan-rich antimicrobial peptide from bovine neutrophils. Int. J. Peptide protein Res., 45: 401-409 (1995).
7. Selsted, M.E., Y.Q. Tang, W.L. Morris, P.A. McGuire, M.J. Novotny, W. Smith, A.H. Henschen, and J.S. Cullor. Purification, primary structures, and antibacterial activities of ß-defensins, a new family of antimicrobial peptides from bovine neutrophils. J. Biol. Chem., 268: 6641-6648 (1993).
8. Tang, Y.Q., and M.E. Selsted. Characterization of the disulfide motif in BNBD-12, an antimicrobial ß-defensin peptide from bovine neutrophils.
J. Biol. Chem., 268: 6649-6653 (1993).
9. Selsted, M.E., M.J. Novotny, W.L. Morris, Y.Q.Tang, W. Smith, and J.S. Cullor. Indolicidin, a novel bacterial tridecapeptide amide from neutrophils. J. Biol. Chem., 267: 4292-4295 (1992).
10. Tang, Y.Q., S.H. Tian, J.C. Hua, S.X. Wu, G. Zou, G.F. Wu, and E.M. Zhao. Isolation, chemical and biological characterization of margaratensin, a neurotensin-related peptide from the skin of Rana margaratae. Science in China (B), 33: 828-834 (1990).
11. Tang, Y.Q., S.H. Tian, J.C. Hua, X.Q. Ji, G.F. Wu, E.M. Zhao, and G. Zou. Isolation and amino acid sequence of a new dodecapeptide from the skin of Oreolalax pingii. Asiatic Herpetological Research, 3: 116-119 (1990).
12. Lu, Y.L., J.L. Peng, Y.Q. Zhu, S.X. Wu, Y.Q. Tang, S.H. Tian, and G. Zou. Synthesis and biological activity of a new frog skin peptide, ranamargarin. Science in China, (B), 33: 170-177 (1990).
13. Tang, Y.Q., J.C. Hua, S.H. Tian, S.X. Wu, J.X. Han, X.Q. Ji, G. Zou, G.F. Wu, and E.M. Zhao. Isolation, structural identification and biological activities of arenobufagin from the skin of Chinese toad Bufa bufa ardrewst. Chinese Pharmaceutical Journal, 25: 138-139 (1990).
14. Tang, Y.Q. High performance liquid chromatography in biochemical research. Part (II). High performance liquid chromatography in peptide chemistry. Chinese J. of Chromatography. 8: 368-372 (1990).
15. Tang, Y.Q., S.H. Tian, S.X. Wu, J.C. Hua, G.F. Wu, E.M. Zhao, Y.A. Lu, Y.Q. Zhu, and G. Zou. Isolation and structure of ranamargarin, a new tachykinin from the skin of Chinese frog Rana margaratae. Science in China, (B), 32: 570-579 (1989).
16. Tang, Y.Q., and F.S. Wang. Molecular weight determination of peptides on the column of Protein I-60 with volatile eluents. Chinese Journal of Chromatography. 4:153-155 (1986).
17. Tang, Y.Q., L.F. Gao, X.X. Qian, and R.B. Zhang. Quantitative analysis of Benserazide by RP-HPLC. Chinese Journal of Chromatography, 2: 56-58 (1985).
18. Tang, Y.Q., J.C. Hua, G. Zou, and E.M. Zhao. A brief review of research on active peptides in amphibian skin. Chinese Acta Herpetologica Sinica, 4: 88-92 (1985).
19. Tsou, K., F.S. Wang, S.H. Wang, and Y.Q. Tang. Dermorphin-like immunoreactivity in guinea pig and rat stomach. Neuropeptides, 5: 449-452 (1985).
20. Tang, Y.Q., J.C. Hua, S.H. Tian, S.X. Wu, G.Y. Hu, X.Q. Ji, G. Zou, G.F. Wu, and E.M. Zhao. Isolation and amino acid sequence of a novel hexapeptide from skin extracts of the Chinese frog Oreolalax pingii. Chinese Acta Herpetologica Sinica, 4: 99-102 (1985).
21. Hua, J.C., S.X. Wu, Y.Q. Tang, W.Z. Zhang, and G. Zou. Isolation and characterization of bradykinin and its two fragments from the skin of the Chinese frog Rana nigromaculata. Chinese Acta Biochimica et Biophysica Sinica, 17: 171-174 (1985).
22. Hua, J.C., Y.Q. Tang, S.H. Tian, S.X. Wu, S.H. Wang, and K. Tsou. Isolation and amino acid sequence of two novel peptides from the skin of the Chinese frog Rana nigromaculata. Chinese Biochemical Journal, 1: 181-182 (1985).
23. Tang, Y.Q. Some experimental aspects of separation of peptides by RP-HPLC. Chinese Chemistry, 5: 31-35 (1984).
24. Tang, Y.Q., and R.B. Zhang. High performance liquid chromatographic analysis of commercial grades of N-phenyl-ß-naphthylamine. Chinese Chemical Worid. 1:13-15 (1982).
25. Tang, Y.Q. and R.B. Zhang. Thermodynamic properties of Trifluoromethyl-1,2,2-triphenylethylenes in reversed-phase high performance liquid chromatography. Chinese Analytical Chemistry, 6: 639-643 (1981).
26. Tang, Y.Q. Synthesis of Sulphinpyrazone. Chinese Pharmaceutical Industry, 10: 8-9 (1981).
1. Tang, Y.Q., M. R. Yeaman, and M. E. Selsted. Antimicrobial peptides from human platelets. Infec. Immun. 70: 6524-6533(2002)
2. Tran, D., P. A. Tran, Y. Q. Tang, J. Yuan, T. Cole, and M. E. Selsted. Homodimeric q-defensins from Rhesus macaque leukocytes. J. Biol. Chem. 277: 3079-3084 (2002).
3 Tang, Y.-Q., J. Yuan, G. Osapay, K. Osapay, D. Tran, C.J. Miller, A.J.
Ouellette and M.E. Selsted. A cyclic antimicrobial peptide produced in primate leukocytes by the ligation of two truncated a-defensins. Science, 286: 498-502 (1999).
4. Tang, Y.Q., J. Yuan, C. J. Miller, and M.E. Selsted. Isolation,
characterization, cDNA cloning and antimicrobial properties of two distinct subfamilies from Rhesus macaque leukocytes. Infec. Immun., 67: 6139-6144 (1999).
5. Yeaman, M.R., Y.Q. Tang, A.J. Shen, A.S. Bayer, and M.E. Selsted. Purification and in vitro avtivities of rabbit platelet microbicidal proteins. Infec. Immun., 65: 1023-1031 (1997).
6. Van Abel, R.J., Y.Q. Tang, V.S.V. Rao, C.H. Dobbs, D. Tran, G. Barany, and M.E. Selsted. Synthesis and characterization of indolicidin, a tryptophan-rich antimicrobial peptide from bovine neutrophils. Int. J. Peptide protein Res., 45: 401-409 (1995).
7. Selsted, M.E., Y.Q. Tang, W.L. Morris, P.A. McGuire, M.J. Novotny, W. Smith, A.H. Henschen, and J.S. Cullor. Purification, primary structures, and antibacterial activities of ß-defensins, a new family of antimicrobial peptides from bovine neutrophils. J. Biol. Chem., 268: 6641-6648 (1993).
8. Tang, Y.Q., and M.E. Selsted. Characterization of the disulfide motif in BNBD-12, an antimicrobial ß-defensin peptide from bovine neutrophils.
J. Biol. Chem., 268: 6649-6653 (1993).
9. Selsted, M.E., M.J. Novotny, W.L. Morris, Y.Q.Tang, W. Smith, and J.S. Cullor. Indolicidin, a novel bacterial tridecapeptide amide from neutrophils. J. Biol. Chem., 267: 4292-4295 (1992).
10. Tang, Y.Q., S.H. Tian, J.C. Hua, S.X. Wu, G. Zou, G.F. Wu, and E.M. Zhao. Isolation, chemical and biological characterization of margaratensin, a neurotensin-related peptide from the skin of Rana margaratae. Science in China (B), 33: 828-834 (1990).
11. Tang, Y.Q., S.H. Tian, J.C. Hua, X.Q. Ji, G.F. Wu, E.M. Zhao, and G. Zou. Isolation and amino acid sequence of a new dodecapeptide from the skin of Oreolalax pingii. Asiatic Herpetological Research, 3: 116-119 (1990).
12. Lu, Y.L., J.L. Peng, Y.Q. Zhu, S.X. Wu, Y.Q. Tang, S.H. Tian, and G. Zou. Synthesis and biological activity of a new frog skin peptide, ranamargarin. Science in China, (B), 33: 170-177 (1990).
13. Tang, Y.Q., J.C. Hua, S.H. Tian, S.X. Wu, J.X. Han, X.Q. Ji, G. Zou, G.F. Wu, and E.M. Zhao. Isolation, structural identification and biological activities of arenobufagin from the skin of Chinese toad Bufa bufa ardrewst. Chinese Pharmaceutical Journal, 25: 138-139 (1990).
14. Tang, Y.Q. High performance liquid chromatography in biochemical research. Part (II). High performance liquid chromatography in peptide chemistry. Chinese J. of Chromatography. 8: 368-372 (1990).
15. Tang, Y.Q., S.H. Tian, S.X. Wu, J.C. Hua, G.F. Wu, E.M. Zhao, Y.A. Lu, Y.Q. Zhu, and G. Zou. Isolation and structure of ranamargarin, a new tachykinin from the skin of Chinese frog Rana margaratae. Science in China, (B), 32: 570-579 (1989).
16. Tang, Y.Q., and F.S. Wang. Molecular weight determination of peptides on the column of Protein I-60 with volatile eluents. Chinese Journal of Chromatography. 4:153-155 (1986).
17. Tang, Y.Q., L.F. Gao, X.X. Qian, and R.B. Zhang. Quantitative analysis of Benserazide by RP-HPLC. Chinese Journal of Chromatography, 2: 56-58 (1985).
18. Tang, Y.Q., J.C. Hua, G. Zou, and E.M. Zhao. A brief review of research on active peptides in amphibian skin. Chinese Acta Herpetologica Sinica, 4: 88-92 (1985).
19. Tsou, K., F.S. Wang, S.H. Wang, and Y.Q. Tang. Dermorphin-like immunoreactivity in guinea pig and rat stomach. Neuropeptides, 5: 449-452 (1985).
20. Tang, Y.Q., J.C. Hua, S.H. Tian, S.X. Wu, G.Y. Hu, X.Q. Ji, G. Zou, G.F. Wu, and E.M. Zhao. Isolation and amino acid sequence of a novel hexapeptide from skin extracts of the Chinese frog Oreolalax pingii. Chinese Acta Herpetologica Sinica, 4: 99-102 (1985).
21. Hua, J.C., S.X. Wu, Y.Q. Tang, W.Z. Zhang, and G. Zou. Isolation and characterization of bradykinin and its two fragments from the skin of the Chinese frog Rana nigromaculata. Chinese Acta Biochimica et Biophysica Sinica, 17: 171-174 (1985).
22. Hua, J.C., Y.Q. Tang, S.H. Tian, S.X. Wu, S.H. Wang, and K. Tsou. Isolation and amino acid sequence of two novel peptides from the skin of the Chinese frog Rana nigromaculata. Chinese Biochemical Journal, 1: 181-182 (1985).
23. Tang, Y.Q. Some experimental aspects of separation of peptides by RP-HPLC. Chinese Chemistry, 5: 31-35 (1984).
24. Tang, Y.Q., and R.B. Zhang. High performance liquid chromatographic analysis of commercial grades of N-phenyl-ß-naphthylamine. Chinese Chemical Worid. 1:13-15 (1982).
25. Tang, Y.Q. and R.B. Zhang. Thermodynamic properties of Trifluoromethyl-1,2,2-triphenylethylenes in reversed-phase high performance liquid chromatography. Chinese Analytical Chemistry, 6: 639-643 (1981).
26. Tang, Y.Q. Synthesis of Sulphinpyrazone. Chinese Pharmaceutical Industry, 10: 8-9 (1981).
Professional Societies
The Protein Society (USA), The Chinese Biochemical Society
Link to this profile
https://faculty.uci.edu/profile/?facultyId=5034
https://faculty.uci.edu/profile/?facultyId=5034
Last updated
10/14/2004
10/14/2004